MXene QDs-Eu³⁺双发射荧光传感构建及其对强力霉素的分析应用

Construction of MXene QDs-Eu³⁺ Dual-Emission Fluorescence Sensor and Its Analytical Application to Doxycycline

摘要: 鉴于四环素类抗生素大量使用造成的环境污染以及人体肝损伤、肠道功能紊乱和过敏等不良反应，亟需开发一种灵敏度高、操作简便、价格低廉的四环素类抗生素残留检测方法。荧光法具有操作简单、成本低廉、可视化等优点，因此，本工作基于MXene QDs和铕离子构建了可选择性检测强力霉素(DOX)的比率荧光探针。以尿酸和单层Ti₃CN为原料通过水热法制备得MXene QDs，MXene QDs与Eu³⁺通过自组装构建得到MXene QDs-Eu³⁺双发射荧光探针，用于DOX的选择性检测。并通过优化探针体系中的MXene QDs浓度、Eu³⁺浓度、pH和响应时间等实验条件，探究该双发射荧光探针对强力霉素的定量和选择性检测性能。结果表明，该荧光探针对DOX具有特异性识别能力，且荧光强度比(F₆₁₅/F₄₂₁)与不同浓度DOX (0∼30 μM)之间存在良好的线性关系，表明其在TCs残留检测中具有良好应用前景。

Abstract: Given the environmental pollution, liver damage, intestinal dysfunction, and allergic reactions caused by the extensive use of tetracycline antibiotics, there is an urgent need to develop a sensitive, simple, and cost-effective method for detecting residual tetracycline antibiotics. The fluorescence method possesses advantages such as simple operation, low cost, and visualizability. Therefore, this work constructs a ratio fluorescence probe for the selective detection of doxycycline based on MXene QDs and rare earth ions. We prepared MXene QDs using uric acid and single-layer Ti₃CN as raw materials through a hydrothermal method. The MXene QDs-Eu³⁺ dual-emission fluorescent probe was constructed by self-assembly of MXene QDs and Eu³⁺, which was used for the selective detection of streptomycin. By optimizing the experimental conditions such as the concentration of MXene QDs, Eu³⁺, pH, and response time in the probe system, this dual-emission fluorescent probe was explored for quantitative and selective detection of streptomycin. Findings show that the fluorescent probe uniquely identifies gentamicin, with a strong linear correlation between the fluorescence intensity ratio (F₆₁₅/F₄₂₁) and different gentamicin concentrations (0~30 μM), hinting at its potential use in identifying tetracycline residues.