红颜草莓组培脱毒快繁体系的建立

Establishment of a Detoxification and Rapid Propagation System for Red Strawberry Tissue Culture

摘要: 以红颜草莓匍匐茎为外植体，采取热处理脱毒和微茎尖脱毒相结合、直接微茎尖脱毒两种脱毒方法，以次氯酸钠为消毒剂，探索不同脱毒方法、消毒时间、消毒浓度对无菌脱毒苗获得的影响，得出在5% NaClO，12 min处理后，直接进行微茎尖脱毒，成活率能达到95%以上且长势最优，有明显生长情况。以MS为基本培养基，探索最佳增殖和生根培养基，增殖培养基为MS + 0.1 mg/L 6-BA + 0.05 mg/L NAA时，增值系数为3.4。生根培养基为1/2 MS + 0.1 mg/L IBA时，生根率能达到100%，无菌脱毒苗移栽到草炭:蛭石:珍珠岩(1:1:1)的栽培基质中成活率为100%。本试验建立红颜草莓组培快繁体系，以期为草莓优良品种规模化繁育和工厂化生产提供技术支撑。

Abstract: Using the creeping stems of red strawberry as explants, two detoxification methods were adopted, including heat treatment detoxification and micro stem tip detoxification, and direct micro stem tip detoxification. Sodium hypochlorite was used as the disinfectant to explore the effects of different detoxification methods, disinfection time, and disinfection concentration on obtaining sterile and detoxified seedlings. It was found that after 12 minutes of treatment with 5% NaClO, direct micro stem tip detoxification resulted in a survival rate of over 95% and optimal growth, with significant growth. Using MS as the basic culture medium, explore the optimal proliferation and rooting culture medium. When the proliferation medium is MS + 0.1 mg/L 6-BA + 0.05 mg/L NAA, the value-added coefficient is 3.4. When the rooting medium is 1/2 MS + 0.1 mg/L IBA, the rooting rate can reach 100%, and the survival rate of sterile and virus-free seedlings transplanted into a cultivation medium of peat:vermiculite:perlite (1:1:1) is 100%. This experiment aims to establish a fast propagation system for red strawberry tissue culture, in order to provide technical support for the large-scale breeding and industrial production of excellent strawberry varieties.