B7-H4 (VTCN1)联合FAS对重度子痫前期患者不良心血管结局的预测价值
The Predictive Value of B7-H4 (VTCN1) Combined with FAS for Adverse Cardiovascular Outcomes in Patients with Severe Preeclampsia
DOI: 10.12677/acm.2024.14102828, PDF,    科研立项经费支持
作者: 董秀娟, 尹艳丽, 徐从从, 许海霞, 张艳霞, 韩丽华, 聂翠翠*：阳光融和医院产科，山东 潍坊
关键词: 子痫前期；B7-H4；FAS；诊断；心血管疾病；Preeclampsia； B7-H4； FAS； Diagnosis； Cardiovascular Disease
摘要: 目的：本研究旨在评估FAS和B7-H4在重度子痫前期(sPE)中的诊断价值及其对围产期不良心血管结局的预测价值。方法：使用GEO2R在线工具分析GSE2347219数据集。收集60例健康孕妇和60名sPE患者，并收集血清。通过RT-qPCR分析血清B7-H4和FAS的表达水平。通过直接ROC分析评估FAS和B7-H4分别对sPE的诊断价值和不良心血管结局的预测价值。通过二元logistics回归分析因子预测概率值，然后进行ROC分析，评估AS和B7-H4联合对sPE的诊断价值和不良心血管结局的预测价值。使用sPE血清培养心肌细胞，检测细胞中的肥大因子和纤维化因子。结果：GSE2347219数据集分析结果显示，FAS和B7-H4在sPE胎盘组织中上调。血清检测结果表明，FAS和B7-H4在sPE血清中上调。直接ROC分析结果表明，FAS和B7-H4可以区分sPE病例与正常孕妇，而且可以预测不良心血管结局。二元logistics回归分析和ROC分析结果表明，B7-H4联合FAS可以有效地区分sPE病例与正常孕妇并有效预测不良心血管结局。sPE血清培养可以升高心肌细胞中的肥大因子(ANP、BNP和β-MHC)和纤维化因子COL1A1的水平。结论：B7-H4 (VTCN1)联合FAS可以作为sPE患者的诊断因子和不良心血管结局的预测因子。
Abstract: Objective: The aim of this study was to evaluate the diagnostic value of FAS and B7-H4 in severe preeclampsia (sPE) and its predictive value for adverse perinatal cardiovascular outcomes. Methods: The GSE2347219 dataset was analyzed using the GEO2R online tool. Sixty healthy pregnant women and sixty patients who were sPE were collected and serum was collected. The expression levels of serum B7-H4 and FAS were analyzed by RT-qPCR. The diagnostic value of FAS and B7-H4 for sPE and the predictive value of adverse cardiovascular outcomes were assessed by direct ROC analysis. Predictive probability values were analyzed by binary logistics regression followed by ROC analysis to assess the diagnostic value and predictive value of adverse cardiovascular outcomes of combined FAS and B7-H4. Cardiomyocytes were cultured using sPE serum, and hypertrophic and fibrotic factors were detected in the cells. Results: Analysis of GSE2347219 dataset showed that FAS and B7-H4 were upregulated in sPE placental tissues. Serum assay results indicated that FAS and B7-H4 were upregulated in sPE serum. Direct ROC analysis showed that FAS and B7-H4 could distinguish sPE cases from normal pregnant women and that they could predict adverse cardiovascular outcomes. Binary logistic regression analysis and ROC analysis showed that B7-H4 in combination with FAS could effectively differentiate sPE cases from normal pregnant women and effectively predict adverse cardiovascular outcomes. sPE serum culture elevated the levels of hypertrophic factors (ANP, BNP, and β-MHC) and fibrosis factor COL1A1 in cardiomyocytes. Conclusion: B7-H4 (VTCN1) in combination with FAS can be used as a diagnostic factor and predictor of adverse cardiovascular outcomes in patients with sPE.
文章引用：董秀娟, 尹艳丽, 徐从从, 许海霞, 张艳霞, 韩丽华, 聂翠翠. B7-H4 (VTCN1)联合FAS对重度子痫前期患者不良心血管结局的预测价值[J]. 临床医学进展, 2024, 14(10): 1531-1538. https://doi.org/10.12677/acm.2024.14102828

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