血清中NSE、S100β、NT-proCNP与大鼠脓毒症相关性脑病的相关性分析

Correlation Analysis of Serum NSE, S100β, NT-proCNP and Rat Sepsis-Associated Encephalopathy

摘要: 目的：探讨神经元特异性烯醇化酶(NSE)、C型利钠肽及其氨基末端前肽(NT-proCNP)和中枢神经特异蛋白(S100β)与脓毒症相关性脑病(SAE)的相关性。方法：SD大鼠共计36只，随机数字表法分为两组(n = 18)，即假手术组(Sham组)和脓毒症相关性脑病组(SAE组)，每组分别在12 h、24 h和48 h进行神经行为学评分并取材。取大鼠心室血离心取上清液，Elisa法检测血清中肿瘤坏死因子-α (TNF-α)、NSE、NT-proCNP、S100β的水平。HE染色观察两组海马组织形态学变化。结果：与Sham组同时间相比，SAE组在24 h和48 h时行为学评分明显降低(SAE组平均数 ± 标准差vs Sham组平均数 ± 标准差，P < 0.05；SAE组平均数 ± 标准差vs Sham组平均数 ± 标准差，P < 0.05)。Elisa法结果显示，相较于Sham组，SAE组TNF-α表达在12 h、24 h和48 h均明显提高(SAE组平均数 ± 标准差vs Sham组平均数 ± 标准差，P < 0.01)，SAE组NSE的表达在24 h和48 h时明显提高(SAE组平均数 ± 标准差vs Sham组平均数 ± 标准差，P < 0.05)；SAE组NT-proCNP的表达在24 h和48 h时明显提高(SAE组平均数 ± 标准差vs Sham组平均数 ± 标准差，P < 0.05)；SAE组S100β的表达在24 h和48 h时明显提高(SAE组平均数 ± 标准差vs Sham组平均数 ± 标准差，P < 0.05)；SAE组内相较于12 h，S100β的表达在24 h和48 h时明显提高(SAE组24 h平均数 ± 标准差vs SAE组48 h平均数 ± 标准差，P < 0.01)。HE结果显示，与Sham组相比，SAE组脑区海马区域组织明显水肿，空泡状增多。结论：大鼠血清中NSE、NT-proCNP和S100β水平的升高与SAE发生具有相关性，动态监测脓毒症患者血清中水平的变化有助于早期发现和诊断SAE。

Abstract: Objective: To explore the correlation between neuron specific enolase (NSE), soluble protein-100β (S100β), amino-terminal pro-C-type natriuretic peptides (NT-proCNP) and sepsis-associated encephalopathy (SAE). Methods: A total of 36 Sprague-Dawley rats were equally distributed into two groups (n = 18), namely the Sham group (undergoing Sham surgery) and the SAE group (with sepsis-associated encephalopathy). At 12 h, 24 h, and 48 h, each group underwent neurobehavioral scoring and tissue collection. The serum was extracted from rat ventricular blood through centrifugation for subsequent measurement of tumor necrosis factor-α (TNF-α), NSE, NT-proCNP, and S100β levels using Elisa. HE staining was employed to examine the structural alterations within the hippocampal tissues of the two groups. Results: Compared with the Sham group, the SAE group showed a significant decrease in neurobehavioral score at 24 h and 48 h (SAE group mean ± SD vs Sham group mean ± SD, P < 0.05; SAE group mean ± SD vs Sham group mean ± SD, P < 0.05). Elisa results showed that compared to the Sham group, TNF-α expression in the SAE group was significantly increased at 12 h, 24 h, and 48 h (SAE group mean ± SD vs Sham group mean ± SD, P < 0.01), NSE expression in the SAE group was significantly increased at 24 h and 48 h (SAE group mean ± SD vs Sham group mean ± SD, P < 0.05); NT-proCNP expression in the SAE group was significantly increased at 24 h and 48 h (SAE group mean ± SD vs Sham group mean ± SD, P < 0.05); S100β expression in the SAE group was significantly increased at 24 h and 48 h (SAE group mean ± SD vs Sham group mean ± SD, P < 0.05); within the SAE group, compared to 12 h, S100β expression was significantly increased at 24 h and 48 h (SAE group 24 h mean ± SD vs SAE group 48 h mean ± SD, P < 0.01). HE staining results showed that compared with the Sham group, the brain region of the hippocampus in the SAE group showed obvious edema and increased vacuolation. Conclusion: The increased levels of NSE, NT-proCNP and S100β in serum of rats are correlated with the occurrence of SAE. Dynamic monitoring of serum levels in sepsis patients is helpful for early detection and diagnosis of SAE.