新型黄芪乳酸益生菌发酵中药产物的毒性试验研究

Experimental Study on Toxicity of Traditional Chinese Traditional Medicine Products Fermented by the New Type of Astragalus membranaceus Lactic Acid Probiotics

摘要: 微生态制剂是利用生物发酵等方式将中药有效成分进行生物学转化并释放出来，该试验研究黄芪乳酸益生菌发酵中药产物作为机体营养增强剂和添加剂的毒理安全性。利用小鼠急性毒性试验、小鼠亚急性毒性试验进行毒理学研究。急性毒性试验表明黄芪乳酸益生菌发酵中药产物对雌雄小鼠的最大耐受量(MTD)均大于35 g/kg∙bw。根据急性毒性试验标准，说明受试动物属于实际无毒级物质。亚急性毒性试验表明5 g/kg∙bw、10 g/kg∙bw、15 g/kg∙bw剂量时，对动物身体、内脏的生长发育、血液生化指标、血常规等均无明显不良影响。黄芪乳酸益生菌发酵中药产物为无毒、绿色、安全、天然产品，可放心的应用于机体营养增强剂及添加剂中。

Abstract: Probiotics is the preparation which uses the methods such as the biological fermentation to bio-logically transform Chinese medicine ingredients and release the Chinese medicine ingredients. The experiment studies the toxicology security of traditional Chinese medicine products which are used to body nutrition enhancers and additives Fermented by Astragalus membranaceus Lactic Acid Probiotics. The toxicology research is characterized by the acute toxicity test and subacute toxicity test in mice. The acute toxicity test shows that the maximum tolerated dose (MTD) of traditional Chinese medicine products fermented by astragalus membranaceus lactic acid probiotics on male and female mice (MTD) is greater than 35 g/kg∙bw. According to the standard of acute toxicity test, the products are nontoxic substances. The subacute toxicity test indicates that when the dose is 5 g/kg∙bw, 10 g/kg∙bw, and 15 g/kg∙bw, there are no significant adverse effects on animal health, growth and development of internal organs, blood biochemical parameters and blood. Traditional Chinese medicine products fermented by astragalus membranaceus lactic acid probiotics are non-toxic, green, safe and natural products, and can be used to body nutrition enhancers and additives.