Masson染色在SD大鼠肺纤维化进展观察中的应用

Application of Masson Stainings in Observation of Pulmonary Fibrosis Progression in SD Rats

摘要: 目的：观察肺纤维化进展组织中Masson染色与HE染色区别。方法：利用气管切开灌注方法构建SD大鼠肺纤维化动态进展模型，分别对肺纤维化组织进行固定、石蜡包埋、HE染色及Masson染色，显微镜观察染色结果。结果：肺泡间隔中胶原纤维呈蓝色，其他纤维呈红色。早期胶原化组织苯胺蓝染色呈浅蓝色，晚期胶原化组织染色呈亮蓝色。结论：Masson染色能提高早期肺纤维化检出率，并易于区分肺纤维化的程度和新旧。

Abstract: Objective: To observe the staining differences between Masson and HE stainings in tissues with progressive pulmonary fibrosis. Methods: A dynamic model of pulmonary fibrosis progression in SD rats was established using tracheal intubation perfusion. Pulmonary fibrosis tissues were fixed, paraffin embedded, stained with HE and Masson, and then observed under a microscope. Results: Collagen fibers in the alveolar septa appeared blue, while other fibers appeared red. In early collagenized tissues, aniline blue staining showed a light blue color, whereas in late collagenized tissues, the staining showed a bright blue color. Conclusion: Masson stainings can improve the detection rate of early pulmonary fibrosis and easily distinguish the degree and age of pulmonary fibrosis.