铁皮石斛花多糖的提取工艺研究
Research on the Extraction Process of Polysaccharides from Dendrobium officinale Flowers
DOI: 10.12677/hjmce.2025.132013, PDF,    科研立项经费支持
作者: 何宇城, 袁建锋*：浙江师范大学行知学院理学院，浙江 金华；马国荣：浙江兰溪锦荣生物科技股份有限公司，浙江 金华
关键词: 铁皮石斛花；多糖；响应面法；Dendrobium officinale Flower； Polysaccharide； Response Surface Methodology
摘要: 为探究铁皮石斛花多糖提取最佳工艺，本研究以铁皮石斛花为研究对象，料液比、超声功率和超声时间为影响因素。在经过单因素实验以确定各个变量的合理范围后，采用了响应面法对提取工艺进行进一步优化。通过实验获得的最佳提取条件为：物料与溶液的比例为1:36.70，超声设备的功率为244.23 W，提取的时间为60.29分钟，铁皮石斛花多糖的提取率可达到6.15%。
Abstract: In order to explore the best extraction process of polysaccharide from Dendrobium officinale flowers, the solid-liquid ratio, ultrasonic power and ultrasonic time were the influencing factors. After single factor experiment to determine the reasonable range of each variable, Box-Behnken response surface method was used to further optimize the extraction process. The optimal extraction conditions were as follows: the ratio of material to solution was 1:36.70, the power of ultrasonic equipment was 244.23W, and the extraction time was 60.29 minutes. Under these conditions, the extraction rate of polysaccharide from Dendrobium officinale flowers could reach 6.15%.
文章引用：何宇城, 马国荣, 袁建锋. 铁皮石斛花多糖的提取工艺研究[J]. 药物化学, 2025, 13(2): 127-135. https://doi.org/10.12677/hjmce.2025.132013

参考文献

[1] He, X., Wang, N., Teng, X., Wang, N., Xie, Z., Dong, Y., et al. (2024) Dendrobium officinale Flowers’ Topical Extracts Improve Skin Oxidative Stress and Aging. Journal of Cosmetic Dermatology, 23, 1891-1904. [Google Scholar] [CrossRef] [PubMed]
[2] Sun, S., Deng, P., Peng, C., Ji, H., Mao, L. and Peng, L. (2022) Extraction, Structure and Immunoregulatory Activity of Low Molecular Weight Polysaccharide from Dendrobium officinale. Polymers, 14, Article 2899. [Google Scholar] [CrossRef] [PubMed]
[3] 胡秦佳宝, 朱亚雄. 铁皮石斛加工制品研究现状[J]. 保鲜与加工, 2019, 19(1): 159-164.
[4] Zhou, J.Y., Sun, M.Y., Deng, J.L., et al. (2019) Effects of Species, Drying and Storage Methods on the Amino Acid Content of Dendrobium officinale Flowers. Food Science and Technology, 44, 88-93.
[5] 张珍林, 韩邦兴, 刘莉彬, 等. ICP-OES测定铁皮石斛干花中多种元素[J]. 中国野生植物资源, 2020, 39(1): 4-8.
[6] Lv, S.H., Xu, M., Zhang, X.F., et al. (2016) Analysis of Polysaccharide, Leachate and Amino Acid Mass Fractions of Dendrobium officinale Flowers from Different Hybrid Family Lines. Journal of Zhejiang A&F University, 33, 749-755.
[7] 霍昕, 周建华, 杨迺嘉, 等. 铁皮石斛花挥发性成分研究[J]. 中华中医药杂志, 2008, 23(8): 735-737.
[8] 曹雪原, 唐雅楠, 姜秀梅, 等. 铁皮石斛不同部位多糖含量及其抗氧化活性研究[J]. 食品与药品, 2018, 20(3): 227-230.
[9] Liang, K.L., Fang, P., Shi, Q.Q., et al. (2018) Antihypertensive Effect and Mechanism of Dendrobium officinale Flowers on High Blood Pressure Rats Induced by High Glucose and High Fat Compound Alcohol. China Journal of Chinese Materia Medica, 43, 147-153.
[10] Li, L., Lei, S., Li, B., Zhou, F., Chen, Y., Su, J., et al. (2020) Dendrobium Officinalis Flower Improves Learning and Reduces Memory Impairment by Mediating Antioxidant Effect and Balancing the Release of Neurotransmitters in Senescent Rats. Combinatorial Chemistry & High Throughput Screening, 23, 402-410. [Google Scholar] [CrossRef] [PubMed]
[11] 雷珊珊, 吕圭源, 金泽武, 等. 铁皮石斛花提取物对甲亢型阴虚小鼠的影响[J]. 中国中药杂志, 2015, 40(9): 1793-1797.
[12] Zhang, W., Liu, X., Sun, X., Han, R., Yu, N., Liang, J., et al. (2022) Comparison of the Antioxidant Activities and Polysaccharide Characterization of Fresh and Dry Dendrobium officinale Kimura Et Migo. Molecules, 27, Article 6654. [Google Scholar] [CrossRef] [PubMed]
[13] Wei, W., Li, Z., Zhu, T., Fung, H., Wong, T., Wen, X., et al. (2017) Anti-Fatigue Effects of the Unique Polysaccharide Marker of Dendrobium officinale on BALB/c Mice. Molecules, 22, Article 155. [Google Scholar] [CrossRef] [PubMed]
[14] 林彦, 段涵怡, 叶敬榕, 等. 微波辅助热水浸提黄芩茎叶多糖的工艺优化[J]. 饲料工业, 2024, 45(10): 106-112.
[15] So, S.H., Lee, J.H., Kim, H.W., Rhee, H.I. and Lee, D.C. (2023) Anti-Inflammatory Effect of Pepper Extract with High Polyamine Levels; Inhibition of ERK/MAPK Pathway in Mice. Food Science and Biotechnology, 33, 677-687. [Google Scholar] [CrossRef] [PubMed]
[16] 于纯淼, 廖贤, 陈小倩, 等. 复合酶法提取诺丽多糖的工艺优化及其体外抗氧化活性[J]. 食品研究与开发, 2025, 46(3): 142-151.
[17] 陈蕾, 郑甜甜, 朱贺宇, 等. 响应面法优化超声辅助提取姬菇多糖及其体外抗氧化性[J]. 中国食品添加剂, 2024, 35(1): 143-149.
[18] 向丽. 复合酶提取桔梗多糖及其抗氧化活性研究[D]: [硕士学位论文]. 绵阳: 西南科技大学, 2021.