辣木提取物的抗氧化和免疫增强作用实验研究
Experimental Study on the Antioxidant and Immune Enhancing Effects of Moringa oleifera Lam Extract
DOI: 10.12677/hjfns.2025.144057, PDF,    科研立项经费支持
作者: 张晓文, 吴 猛*, 陈 晓, 蔡绮君：广州市体育科学研究所运动营养室，广东 广州；宋 清：广州体育职业技术学院体育康复医院，广东 广州；杨 倩, 黄儒强：华南师范大学生命科学院，广东 广州
关键词: 辣木；抗氧化；免疫增强作用；NK细胞活性；Moringa oleifera Lam； Antioxidant； Immune Enhancement Effect； NK Cell Activity
摘要: 目的：探讨辣木提取物的抗氧化和免疫增强作用，为其抗运动疲劳领域的应用提供科学依据。方法：1) 抗氧化指标：选用雄性SD大鼠建立高血脂动物模型，测定辣木提取物对大鼠肝脏总超氧化物歧化酶(T-SOD)和丙二醛(MDA)水平。2) 免疫功能指标：通过免疫重量法检测对KM小鼠免疫器官胸腺、脾脏重量的影响；采用脾淋巴细胞增殖活性及小鼠脾淋巴细胞转化实验、NK细胞活性测定实验，观察辣木提取物对小鼠细胞免疫功能的影响。结果：1) 抗氧化实验：辣木提取物中剂量组T-SOD酶活性显著高于对照组，MDA酶活性显著低于对照组(均p < 0.05)，表明其明显提高T-SOD酶活性和降低MDA酶水平。2) 免疫功能指标：免疫重量法检测结果显示，辣木提取物各组脾脏指数、胸腺指数均较Cy模型对照组明显增加(p < 0.05)，其中高剂量组增加尤其显著。辣木提取物各剂量组显著增加小鼠脾淋巴细胞转化率，且作用呈浓度依赖性。同时辣木提取物各组NK细胞活性均较Cy模型组显著提高，最高增加幅度达47.92% (p < 0.01)，表明具有明显提高小鼠脾脏Ｔ淋巴细胞转化功能及NK细胞杀伤活性。结论：辣木提取物具有显著的抗氧化和免疫增强作用，为其在抗疲劳领域的开发利用提供了理论依据。
Abstract: Objective: To explore the effect of antioxidant and immune-enhancing properties of Moringa oleifera extract and to investigate its application value and scientific basis in anti-fatigue. Methods: 1) Antioxidant indicators: Male SD rats were used to establish a hyperlipidemia animal model, and the levels of total superoxide dismutase (T-SOD) and malondialdehyde (MDA) in the liver of rats were measured after administration of Moringa oleifera extract. 2) Immune function indicators: KM mice were used, and the spleen and thymus indices of each group of mice were detected by the immune weight method, and the effect of Moringa oleifera extract on the cellular immune function of mice was observed. NK cell activity was also measured. Results: 1) Antioxidant experiment: The T-SOD enzyme activity in the medium-dose group of Moringa oleifera extract was significantly higher than that in the control group (p < 0.05), and the MDA enzyme level was significantly lower than that in the control group (p < 0.05), indicating a significant increase in T-SOD enzyme activity and a decrease in MDA enzyme activity. 2) Immune function indicators: The spleen index of each dose group was detected by the immune weight method. The spleen index and thymus index of each dose Moringa oleifera extract group were increased significantly compared with the Cy model control group (p < 0.05). The high-dose group of Moringa oleifera extract could enhance the NK cell activity of mice by up to 47.92% (p < 0.05), which indicates a significant improvement in the transformation function of T lymphocytes in the spleen and the killing activity of NK cells in mice. Conclusion: Moringa oleifera extract has significant antioxidant and immune-enhancing effects, providing a theoretical basis for its in-depth development in the field of anti-fatigue.
文章引用：张晓文, 宋清, 吴猛, 杨倩, 黄儒强, 陈晓, 蔡绮君. 辣木提取物的抗氧化和免疫增强作用实验研究[J]. 食品与营养科学, 2025, 14(4): 519-526. https://doi.org/10.12677/hjfns.2025.144057

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