响应面法研究藜麦中多酚的提取工艺

Study on Extraction Technology of Polyphenols from Quinoa by Response Surface Method

摘要: 由于藜麦中多酚具有抗氧化、抑制炎症反应、预防心脑血管疾病等多种健康功效，具有较高的研究价值。本研究以多酚作为对象，通过单因素试验得到结果，利用响应面软件分析数据，从而探究藜麦中多酚提取工艺的最佳条件，实验采用回流和有机溶剂法对藜麦中的多酚进行提取，从料液比、提取液与乙醇比、乙醇的体积分数三方面分别进行单因素试验。最终得出提取工艺的最佳条件为：料液比1:45 g/mL、提取液与乙醇比1:6 mL、乙醇的体积分数95%。在此条件下，得到多酚的最佳提取率为0.4146%。为今后对于藜麦中多酚的提取提供了参考方法，能够得到较高的提取率。

Abstract: Because the polyphenols in quinoa have many health effects such as anti-oxidation, inhibition of inflammatory response, prevention of cardiovascular and cerebrovascular diseases, it has high research value. In this study, polyphenols were used as the object, and the results were obtained by single-factor test. The response surface software was used to analyze the data, so as to explore the optimum conditions for the extraction process of polyphenols in quinoa. The polyphenols in quinoa were extracted by reflux and organic solvent method, and the single-factor test was carried out from three aspects: the ratio of material to liquid, the ratio of extract to ethanol, and the volume fraction of ethanol. Finally, the optimum conditions for the extraction process were as follows: the ratio of material to liquid was 1:45 g/mL, the ratio of extract to ethanol was 1:6 mL, and the volume fraction of ethanol was 95%. Under these conditions, the optimum extraction rate of polyphenols was 0.4146%. It provides a reference method for the extraction of polyphenols in quinoa in the future, and can obtain a higher extraction rate.