柱前衍生–高效液相色谱法测定肉类中氰化物含量的研究
A Study on the Determination of Cyanide in Meat by Pre-Column Derivatization-High Performance Liquid Chromatography
DOI: 10.12677/hjfns.2025.146092, PDF,   
作者: 吴余欣, 黄 南, 钟世欢, 项睿洁, 叶佳明, 王 京, 潘雷明*：浙江公正检验中心有限公司，浙江 杭州；蒋 晗：中国计量大学生命科学学院，浙江 杭州；王馨远：杭州市食品药品检验科学研究院食品检验研究所，浙江 杭州
关键词: 氰化物；高效液相色谱法；异烟酸–吡唑啉酮；柱前衍生；Cyanide； HPLC； Isonicotinic Acid-Pyrazoline； Pre-Column Derivatization
摘要: 建立柱前衍生–高效液相色谱法测定肉类中氰化物含量的检测方法。样品经水蒸气蒸馏，采用异烟酸–吡唑啉酮柱前衍生，高效液相色谱法测定。采用迪马Silversil C18 (4.6 mm × 250 mm, 5 μm)色谱柱，以0.02 mol/L乙酸铵–甲醇(65:35)为流动相。检测波长638 nm。氰化物的衍生产物在0.02~110 mg/kg这一浓度范围里线性状况良好，其相关系数超过0.999，检出限为0.02 mg/kg。猪肉、鸭肉、狗肉中氰化物的平均回收率均大于95%。
Abstract: A method for determining the cyanide content in meat using pre-column derivatization-high-performance liquid chromatography was established. The sample was subjected to steam distillation, and the pre-column derivatization was performed using isonicotinic acid-pyrazoline. The cyanide derivative was determined by high-performance liquid chromatography. The Silversil C18 (4.6 mm × 250 mm, 5 μm) column from Dima was used, and the mobile phase was acetate ammonium-methanol (65:35). The detection wavelength was 638 nm. The linear relationship was good within the concentration range of 0.02~110 mg/kg, with a correlation coefficient of over 0.999. The detection limit was 0.02 mg/kg. The average recovery rates of cyanide in pork, duck, and dog meat were all greater than 95%.
文章引用：吴余欣, 黄南, 钟世欢, 项睿洁, 叶佳明, 王京, 蒋晗, 王馨远, 潘雷明. 柱前衍生–高效液相色谱法测定肉类中氰化物含量的研究[J]. 食品与营养科学, 2025, 14(6): 837-845. https://doi.org/10.12677/hjfns.2025.146092

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