益生菌GKM3对肥胖患者的减重效果评估

Evaluation of the Weight Loss Effect of Probiotic GKM3 in Obese Patients

摘要: 益生菌被定义为活体微生物，当以足够的剂量施用时，可赋予宿主健康益处。本研究利用人类结肠类癌细胞株NCI-H716细胞株评估，发现Lactobacillus plantarum GKM3培养上清液可促进其分泌GLP-1蛋白，可能有助于抗肥胖，因此进行评估L. plantarum GKM3能否对肥胖患者产生有益性的效果。28位受试者于每日早、晚餐后各服用一次含L. plantarum GKM3的益生菌胶囊，连续90天，要求受试者每日记录体重变化，每周记录体脂肪变化，并在服用前第1天、第45天以及第90天采集血液，评估服用益生菌前后的血液生化数值变化。结果发现服用L. plantarum GKM3后，受试者于试验结束时体脂率显著下降(p < 0.05)，而体重与BMI虽有下降趋势，但未达显著。以BMI将受试者分组后发现，过重组于第45天及第90天均显著降低体重及BMI (p < 0.05)，中度肥胖组体脂率于第90天显著下降(p < 0.05)。血脂指标方面，高密度脂蛋白胆固醇(HDL-C)显著上升(p < 0.05)，总胆固醇与HDL-C比值亦显著改善。血糖、肝肾功能及甲状腺指数无显著变化。此外观测受试者排便情形，服用L. plantarum GKM3后，确实可明显地改善腹泻情形。综合结果显示，L. plantarum GKM3可促进GLP-1分泌，并对体脂调控、血脂代谢及肠道健康具有正向影响，具有作为辅助体重管理的潜力。

Abstract: Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer health benefits on the host. In this study, the human enteroendocrine cell line NCI-H716 was used to evaluate the effects of Lactobacillus plantarum GKM3, and the results demonstrated that the culture supernatant of L. plantarum GKM3 promoted GLP-1 secretion, suggesting a potential anti-obesity effect. To further investigate, we conducted a human trial in which 28 participants consumed one capsule containing L. plantarum GKM3 after breakfast and dinner daily for 90 consecutive days. Participants were instructed to record their body weight daily, measure body fat once per week, and provide blood samples on Day 1, Day 45, and Day 90 for biochemical analysis. The results showed that after supplementation with L. plantarum GKM3, body fat percentage was significantly reduced at the end of the trial (p < 0.05), whereas reductions in body weight and BMI did not reach statistical significance. Subgroup analysis by BMI revealed that the overweight group exhibited significant reductions in both body weight and BMI on Day 45 and Day 90 (p < 0.05), while the moderately obese group showed a significant reduction in body fat percentage at Day 90 (p < 0.05). Regarding lipid profiles, high-density lipoprotein cholesterol (HDL-C) was significantly increased (p < 0.05), and the total cholesterol-to-HDL-C ratio was significantly improved. No significant changes were observed in blood glucose, liver and kidney function, or thyroid indices. In addition, bowel habit assessments indicated that supplementation with L. plantarum GKM3 markedly reduced the incidence of diarrhea. Taken together, these findings demonstrate that L. plantarum GKM3 promotes GLP-1 secretion and exerts beneficial effects on body fat regulation, lipid metabolism, and gut health, supporting its potential as an adjunct for weight management.