羊肚菌多糖对人结肠癌细胞SW620体外增殖的抑制作用

Inhibitory Effect of Morchella Polysaccharide on the Proliferation of Human Colon Cancer Cell Line SW620 in Vitro

摘要: 目的：研究羊肚菌多糖对人结肠癌细胞SW620体外增殖的抑制作用。方法：采用mtt法、划线法和集落生成分析法检测羊肚菌多糖对人结肠癌细胞SW620的体外增殖抑制作用，采用流式细胞技术检测羊肚菌多糖对人结肠癌细胞SW620的凋亡作用，采用western blotting技术检测相关凋亡蛋白的表达情况。结果：1) 羊肚菌多糖对人结肠癌细胞SW620的体外增殖、细胞迁移、细胞集落形成具有抑制作用，其作用24 h的IC₅₀为1220 ± 50 μg/mL，48 h的IC₅₀为968 ± 63 μg/mL；2) 羊肚菌多糖通过凋亡途径抑制SW620细胞的增殖；3) 在羊肚菌多糖的作用下抗凋亡蛋白Bcl-2的表达下降，而凋亡蛋白Bax和caspase-3的表达上升。结论：羊肚菌多糖能显著抑制人结肠癌细胞SW620的增殖，并通过上调Bax和caspaes-3的表达和下调Bcl-2的表达诱导SW620细胞凋亡。

Abstract: Objective: To study the inhibitory effect of Morchella polysaccharide on human colon cancer cell line SW620 in vitro. Methods: MTT assay, scratch wound-healing and colony-forming analysis were used to detect the inhibitory effect of Morchella polysaccharide on the proliferation of human colon cancer cell SW620 in vitro, flow cytometry was used to detect the apoptosis effect of Morchella polysaccharide on human colon cancer cell SW620, and western blotting was used to detect the expression of related apoptosis proteins. Results: 1) Morchella polysaccharide treatment resulted in inhibition of proliferation, migration and colony formation of SW620 cancer cell in a time and dose-dependent, and the IC₅₀ of Morchella polysaccharide is 1220 ± 50 μg/mL for 24h and 968 ± 63 μg/mL for 48 h; 2) Morchella polysaccharide inhibits the proliferation of SW620 cells through the apoptotic pathway; 3) Morchella polysaccharide treatment-induced SW620 cancer cell apoptosis through up-regulated the expression of caspase-3 and Bax and down-regulated Bcl-2 levels. Conclusion: Morchella polysaccharide is a potent inhibitor of colon cancer growth by induction of SW620 cell apoptosis through intrinsic apoptosis pathway.