基于近红外光谱结合化学计量学的银杏酮酯喷干粉中的总黄酮醇苷的检测研究
Research on the Determination of Total Flavonol Glycosides in Ginkgo Ester Spray Powder Based on Near Infrared Spectroscopy and Chemometrics
摘要: 目的：本研究旨在建立近红外光谱法快速检测银杏酮酯喷干粉中总黄酮醇苷的方法。方法：利用化学计量学中PCA方法，结合霍特林T检验算法判定采集到样品的近红外光谱是有效的，使用偏最小二乘法(partial least squares, PLS)建立银杏酮脂喷干粉中总黄酮醇苷的快速检测方法。结果：根据Hotelling’s T2判定结果，采集的63批样品光谱数据无异常值，符合后续建模要求。使用模型评价方法对模型的稳定性、拟合程度、准确性进行考察，符合模型建立要求。结论：上述方法可用于银杏酮酯喷干粉的总黄酮醇苷的快速检测。
Abstract: Objective: The aim of this study was to establish a method for rapid determination of total flavonoid glycosides in ginkgo ester spray powder by near infrared spectroscopy. Methods: The PCA method in chemometrics combined with the Hotelling T test algorithm was used to determine the validity of the near infrared spectrum of samples collected. Partial least squares (PLS) was used to establish a rapid detection method for total flavonol glycosides in ginkgo ester spray powder. Results: According to Hotelling’s T2 judgment results, the collected spectral data of 63 batches of samples had no normal values, which met the requirements of subsequent modeling. The stability, fitting degree and accuracy of the model were investigated by using the model evaluation method, which met the requirements of model establishment. Conclusion: The method can be used for the rapid determination of total flavonol glycosides in ginkgo ester spray powder.
文章引用：黄建强, 朱宝中, 王金峰. 基于近红外光谱结合化学计量学的银杏酮酯喷干粉中的总黄酮醇苷的检测研究[J]. 药物化学, 2024, 12(4): 297-304. https://doi.org/10.12677/hjmce.2024.124033

参考文献

[1] 国家药典委员会. 中国药典2020年版(一部) [M]. 北京: 中国医药科技出版社, 2020: 329-330.
[2] Tang, Z., Jiang, D.Z., Zhao, X.X., et al. (2020) Comparison of Different Specifications of Ginkgo Biloba Extract and Discussion on Specification Improvement. Drug Stand China, 21, 21-25.
[3] Ke, J., Li, M., Huo, Y., Cheng, Y., Guo, S., Wu, Y., et al. (2021) The Synergistic Effect of Ginkgo Biloba Extract 50 and Aspirin against Platelet Aggregation. Drug Design, Development and Therapy, 15, 3543-3560. [Google Scholar] [CrossRef] [PubMed]
[4] Li, L., Zhao, Y., Du, F., Yang, J., Xu, F., Niu, W., et al. (2012) Intestinal Absorption and Presystemic Elimination of Various Chemical Constituents Present in GBE50 Extract, a Standardized Extract of Ginkgo Biloba Leaves. Current Drug Metabolism, 13, 494-509. [Google Scholar] [CrossRef] [PubMed]
[5] Luo, Y. and Smith, J.V. (2004) Studies on Molecular Mechanisms of Ginkgo Biloba Extract. Applied Microbiology and Biotechnology, 64, 465-472. [Google Scholar] [CrossRef] [PubMed]
[6] Smith, J.V. and Luo, Y. (2003) Elevation of Oxidative Free Radicals in Alzheimer’s Disease Models Can Be Attenuated by Ginkgo Biloba Extract EGb 761. Journal of Alzheimer’s Disease, 5, 287-300. [Google Scholar] [CrossRef] [PubMed]
[7] Fan, L.C., Zhou, L.C. and An, C.H. (2018) Inhibitory Effect of Total Ginkgo Flavonoids on Lipopolysaccharide-Induced Inflammatory Response in Microglial Cells. International Journal of Neurology and Neurosurgery, 45, 477-480.
[8] 李文龙, 瞿海斌. 近红外光谱应用于中药质量控制及生产过程监控的研究进展[J]. 浙江大学学报(医学版), 2017, 46(1): 80-88.
[9] 倪鸿飞, 斯乐婷, 黄家鹏, 等. 近红外光谱结合遗传算法优化的极限学习机实现银杏叶纯化过程有效成分快速测定[J]. 中国中药杂志, 2021, 46(1): 110-117.
[10] 王超, 李朋成, 杨凯, 等. 近红外光谱烟叶质量等级快速检测与等级特征分析[J]. 光谱学与光谱分析, 2021, 41(3): 943-947.
[11] 徐杰, 陈海宏, 杨清, 等. 红外光谱法测定黏胶/氨纶织物的纤维含量[J]. 上海纺织科技, 2021, 49(2): 54-56.
[12] 石培华. 利用近红外光谱技术快速测定航空煤油物理性质[J]. 石化技术, 2021, 28(1): 1-3.
[13] 白丽萍, 王伟, 王强, 等. 近红外光谱快速检测葡萄酒品质[J]. 浙江农业科学, 2021, 62(2): 389-391, 400.
[14] 石岩, 刘薇, 魏锋, 等. 基于化学计量学的川贝母药材及其混伪品平贝母的鉴别方法的建立[J]. 药物分析杂志, 2024, 44(7): 1222-1232.