标题:
羽扇豆酮提取物的含量测定Determination of Lupenone Extract
作者:
牟珍, 王远敏, 吴红梅, 王祥培
关键字:
芭蕉根, 羽扇豆酮, 高效液相色谱, 含量测定Rhizoma Musae, Lupenone, HPLC, Determination
期刊名称:
《Pharmacy Information》, Vol.5 No.4, 2016-11-17
摘要:
目的：测定从芭蕉根中分离的羽扇豆酮提取物的含量，鉴定其纯度，为羽扇豆酮提取物抗糖尿病新药的开发奠定基础。方法：以甲醇-乙腈 = 1:1 (V:V)单泵作为流动相，柱温25℃，波长206 nm，流速1 mL/min，Diamonsi-C18 (4.6 mm × 250 mm, 5 μm)。结果：分离得到的三批羽扇豆酮提取物中羽扇豆酮的含量分别为98.27%、97.40%、99.29%。结论：该方法简单、方便、快速，可以用于羽扇豆酮提取物的含量测定及质量控制，同时三批羽扇豆酮提取物的纯度均达到97%以上，可以用于羽扇豆酮抗糖尿病新药的开发研究。
Objective: In order to provide the foundation for the development of lupenone used as a new an-ti-diabetic drug, we determined the content and purity of lupenone extract from Rhizoma Musae. Methods: Methanol-acetonitrile = 1:1 (V:V) single pump as the mobile phase, column temperature 25˚C, the wavelength of 206 nm, flow rate 1 mL/min, Diamonsi-C18 (4.6 mm × 250 mm, 5 μm). Results: Three batches of lupenone extract isolated in lupenone contents were 98.27%, 97.40%, 99.29%. Conclusion: The method is simple, convenient, fast and can be used for the determination and quality control and lupenone extract. At the same time, the purity of three batches of lupenone extract were more than 97%, so lupenone can be developed into a new anti-diabetic drug.